Kinetika oksidacije aldehida katalizirana aldehid dehidogenazom

undergraduate thesis

• Title (english): Kinetics of aldehyde oxidation catalyzed by aldehyde dehydrogenase
• Author: Anamarija Volf
• Mentor: Zvjezdana Findrik Blažević (mentor)
• Committee member: Zvjezdana Findrik Blažević (predsjednik povjerenstva)
• Committee member: Martina Sudar (član povjerenstva)
• Committee member: Marija Vuković Domanovac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2017-09-13, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering; Reaction Engineering

Abstract

U ovom je radu provedena enzimatska oksidacija dvaju aldehida: formaldehida (FA) i propanala (PA) uz prisutstvo enzima aldehid dehidrogenaze (ALDH). Aldehid dehidrogenaza je enzim koji za svoje katalitičko djelovanje treba prisutstvo koenzima NAD+. NAD+ se obnavlja regeneracijom uz pomoć enzima NADH oksidaze (NOX). Određena je kinetika reakcije oksidacije aldehida katalizirana aldehid dehidrogenazom. Ispitan je utjecaj promjene koncentracije supstrata (FA/PA i NAD+) te produkta (NADH) na početnu reakcijsku brzinu. Uz pomoć eksperimentalnih podataka procijenjeni su kinetički parametri Michaelis-Menteničinog modela koji je kasnije validiran eksperimentom u kotlastom reaktoru za oksidaciju propanala.

Abstract (english)

Enzymatic oxidation of two aldehydes: formaldehyde (FA) and propionaldehyde (PA) in presence of enzyme aldehyde dehydrogenase (ALDH) was studied in this work. Aldehyde dehydrogenase is an enzyme which requires coenzyme NAD+ for its catalytic activity. NAD+ is regenerated by enzyme NADH oxidase (NOX). Kinetic model of aldehyde oxidation catalyzed by aldehyde dehidrogenase was developed. The influence of concentration of substrates (FA/PA and NAD+) and product (NADH) on the initial reaction rate was examined. Kinetic parameters of the Michaelis-Menten model were estimated from the experimental data. The mathematical model was experimentally validated in the batch reactor for propanal oxidation.

• Keywords: enzimatska oksidacija; aldehid; enzim; koenzim; kinetika; kotlasti reaktor
• Keywords (english): enzymatic oxidation; aldehyde; enzyme; coenzyme; kinetics; batch reactor
• Language: croatian
• URN:NBN: urn:nbn:hr:149:545891
• Project: Number: 635595; Title: Sustainable industrial processes based on a C-C bond-forming enzyme platform; Acronym: CARBAZYMES; Leader: Zvjezdana Findrik Blažević; Jurisdiction: eu; Funder: EC; Funding stream: H2020
• Study programme: Title: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Environmental chemistry and green technologies; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-03-11 11:20:41