Primjena niskotemperaturnih eutektičkih otapala za ekstrakciju polifenola iz biljnog materijala

master's thesis

• Title (english): Application of deep eutectic solvents for the extracion of polyphenols from plant material
• Author: Marijana Perković
• Mentor: Marko Rogošić (mentor)
• Committee member: Marko Rogošić (predsjednik povjerenstva)
• Committee member: Aleksandra Sander (član povjerenstva)
• Committee member: Sanda Vladimir-Knežević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2019-06-18, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

U ovom radu cilj je odrediti jesu li odabrana niskotemperaturna eutektička otapala bolja za ekstrakciju polifenola iz biljnog materijala od najčešće korištenih konvencionalnih otapala. Konvencionalna otapala koja pronalaze uporabu u različitim industrijama nastoje se zamijeniti zbog njihove hlapljivosti, toksičnosti, zapaljivosti i cijene. Iz dvije različite biljke (bazga i ružmarin), odnosno iz dva različita dijela tih biljaka (usitnjeni cvijet bazge i usitnjeni list ružmarina) ekstrahirane su odabrane skupine polifenola (fenolne kiseline i flavonoidi) po propisima Europske farmakopeje te su kvantitativno određene spektrofotometrijskom metodom. Prisutnost fenolnih kiselina i flavonoida potvrđena je tankoslojnom kromatografijom. Osam različitih otapala uspoređeno je s obzirom na ekstrakciju polifenolnih spojeva (destilirana voda, 70 %-tni etanol, 100 % ChCl:EG = 1:3, 70 % ChCl:EG = 1:3, 50 % ChCl:EG = 1:3, 100 % ChCl:Gly = 1:3, 70 % ChCl:Gly = 1:3, 50 % ChCl:Gly = 1:3) Za razliku od konvencionalnih otapala, niskotemperaturna eutektička otapala na bazi kolinklorida, etilen-glikola i glicerola odabrana za ovaj diplomski rad pripadaju skupini zelenih otapala. Ispitivan je utjecaj proton-donorske skupine za ekstrakciju polifenolnih spojeva. Rezultati ovog istraživanja pokazala su da primijenjena niskotemperaturna eutektička otapala s 50 % sadržajem vode vrlo dobro ekstrahiraju fenolne kiseline kao što su ružmarinska i klorogenska kiselina, dok sva korištena otapala zanemarivo malo ekstrahiraju flavonoide iz biljaka u usporedbi sa 70 %-tnim etanolom.

Abstract (english)

The main objective of this thesis is to determine if selected deep eutectic solvents are better for the extraction of polyphenols from herbal material than most commonly used conventional solvents. Conventional solvents which are used in many different industries are attempted to be replaced because of their volatility, toxicity, flammability and price. The selected groups of polyphenols (phenolic acids and flavonoids) were extracted from two different herbs (elderberry and rosemary), i.e. from two different parts of those herbs (milled dry elderberry flowers and milled dry rosemary leaves) according to European pharmacopoeia regulations and quantitatively determined by the spectrophotometric method. The presence of phenol acids and flavonoids is confirmed by the thin layer chromatography Eight different solvents were compared considering the extraction of polyphenol compounds (distilled water, 70 % ethanol, 100 % ChCl:EG = 1:3, 70 % ChCl:EG = 1:3, 50 % ChCl:EG = 1:3, 100 % ChCl:Gly = 1:3, 70 % ChCl:Gly = 1:3, 50 % ChCl:Gly = 1:3). Unlike conventional solvents, deep eutectic solvents based on choline chloride, ethylene glycol and glycerol selected for this thesis belong to the group of green solvents. The impact of proton donor group on the extraction of polyphenol compounds is examined. The results of this study show that the deep eutectic solvents with 50 % water content are very efficient in extracting phenolic acids such as rosemary acid and chlorogenic acid, while all used solvents exhibit negligible extraction of flavonoids from plants in comparison with 70 % ethanol.

• Keywords: DES; konvencionalna otapala; zelena otapala; ekstrakcija; fenolne kiseline; flavonoidi
• Keywords (english): DES; conventional solvents; green solvents; extraction; phenolic acids; flavonoids
• Language: croatian
• URN:NBN: urn:nbn:hr:149:996849
• Project: Number: IP-2014-09-9550; Title: Zelena otapala za zelene tehnologije; Title: Green Solvents for Green Technologies; Acronym: GREEN; Leader: Ivana Radojčić Redovniković; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-02-26 21:03:53