Primjena punog faktorskog plana i metode odzivnih površina za određivanje učinka mikroplastike na stanične linije zdravog i tumorskog porijekla

master's thesis

• Title (english): Application the Full Factorial Plan and Response Surface Methodology to determine the effect of microplastics on healthy and tumor cell lines
• Author: Iva Polonji
• Mentor: Dajana Kučić Grgić (mentor)
• Mentor: Jelena Knežević (komentor)
• Committee member: Dajana Kučić Grgić (predsjednik povjerenstva)
• Committee member: Šime Ukić (član povjerenstva)
• Committee member: Matija Cvetnić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2022-09-16, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Pretjerana uporaba plastičnih materijala i loše gospodarenje plastičnim otpadom stvorili su rastući problem u pogledu onečišćenja okoliša te utjecaja na žive organizme koji u njemu žive, pa tako i čovjeka. Poseban naglasak se u sklopu novijih znanstvenih istraživanja stavlja na mikroplastiku, koja nastaje kao posljedica fragmentacije plastičnog otpada uslijed izlaganja okolišnih uvjetima. Ova vrsta plastike predstavlja specifičnu opasnost, budući da se zbog svoje veličine olakšano kreće kroz ekosustave, uklapa u hranidbene lance i akumulira u živim jedinkama na koje potom ima potencijalno štetne učinke. U ovome radu provedeno je ispitivanje učinka mikročestica polistirena na relativno preživljenje humanih plućnih staničnih linija zdravog (WI-38) i tumorskog porijekla (H1299 i A549) u ovisnosti o rasponu veličina čestica mikroplastike, koncentraciji otopina mikroplastike kojim su stanice tretirane i vremenu izlaganja. Kako bi se ustanovilo relativno preživljenje testnih staničnih kultura proveden je MTT test, a programom Desing Expert 7.1.5. određeni su eksperimentalni uvjeti kojima je postignut najštetniji učinak mikroplastike na ispitne stanične linije, odnosno uvjeti pri kojima je relativno preživljenje stanica bilo najniže. Za staničnu liniju WI-38 minimalno preživljenje ustanovljeno je pri: 157,01 μm (veličina čestica PS-a), 0,50 μg/mL (koncentracija otopina PS-a) i 39,74 sata (vrijeme izlaganja), a značajni procesni parametri bili su koncentracija i veličina čestica. Za H1299 najniže preživljenje ostvarilo se pri: 103,11 μm (veličina čestica PS-a), 1,00 μg/mL (koncentracija otopina PS-a) i 24 sata (vrijeme izlaganja), pri čemu su značajni procesni parametri bili koncentracija i vrijeme izlaganja. Stanična linija A549 najmanje preživljenje imala je pri: 200 μm (veličina čestica PS-a), 2,00 μg/mL (koncentracija otopina PS-a) i 51,17 sata (vrijeme izlaganja), dok je značajni procesni parametar bio isključivo vrijeme. Prema dobivenim rezultatima zaključeno je da štetan učinak mikroplastike PS-a u pogledu smanjenja relativnog preživljenja postoji, te da s obzirom na vrstu ispitne stanične linije može ovisiti o veličini čestica PS-a, koncentraciji otopina i vremenu izlaganja.

Abstract (english)

Excessive use of plastic materials and poor management of plastic waste in modern times have created a growing problem in terms of environmental pollution and impact on living organisms that live in it, including humans. As part of recent scientific research, special emphasis is placed on microplastics, which are formed as a result of the fragmentation of plastic waste due to exposure to environmental conditions. This type of plastic represents a specific danger, since due to its size it can easily move through ecosystems, fit into food chains and accumulate in living organisms, on which it can have potentially harmful effects. In this work, the effect of polystyrene microparticles on the relative survival of human lung cell lines of healthy origin (WI-38) and tumor origin (H1299 and A549) was investigated. The dependence of survival on the size range of the microplastic particle, the concentration of the microplastic solution with which the cells were treated and the exposure time was observed. In order to establish the relative survival of the test cell cultures, the MTT test was performed. Program Desing Expert 7.1.5. was used to determine the experimental conditions under which the most harmful effect of microplastics on the test cell lines was achieved. In other words, those were the conditions under which relative cell survival was the lowest. For the WI-38 cell line, minimum survival was found at: 157.01 μm (PS particle size), 0.50 μg/mL (PS solution concentration) and 39.74 hours (exposure time), a significant process parameters were concentration and particle size. For H1299, the lowest survival was achieved at: 103.11 μm (PS particle size), 1.00 μg/mL (PS solution concentration) and 24 hours (exposure time), where the significant process parameters were concentration and exposure time. The A549 cell line had the lowest survival at: 200 μm (PS particle size), 2.00 μg/mL (PS solution concentration) and 51.17 hours (exposure time), while the only significant process parameter was time. According to the obtained results, it was concluded that the harmful effect of PS microplastics in terms of reducing relative survival exists. Also, depending on the test cell culture type, the decrease in survival depends on the size of the PS particle, the concentration of the solution and the exposure time.

• Keywords: mikroplastika; polistiren; WI-38; H1299; A549
• Keywords (english): microplastics; polystyrene; WI-38; H1299; A549
• Language: croatian
• URN:NBN: urn:nbn:hr:149:974486
• Project: Number: IP-2019-04-9661; Title: Primjena naprednih tehnologija obrade voda za uklanjanje mikroplastike; Title: Advanced Water Treatment Technologies for Microplastics Removal; Acronym: AdWaTMiR; Leader: Tomislav Bolanča; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Project: Number: IP-2016-06-1441; Title: Genetički i epigenetički biomarkeri urođene imunosti u KOPB-u i karcinomu pluća; Acronym: LungInflaCare; Leader: Jelena Knežević; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Environmental chemistry and green technologies; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra primijenjene kemije (magistar/magistra primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-06-01
• Created on: 2023-03-19 21:02:10