Metode razgradnje, izolacije i identifikacije mikroplastike prisutne u sadržaju probavila riba

master's thesis

• Title (english): Methods of digestion, isolation and identification of microplastics present in the fish gut content
• Author: Dubravka Datković
• Mentor: Dajana Kučić Grgić (mentor)
• Mentor: Vlatka Filipović Marijić (komentor)
• Committee member: Dajana Kučić Grgić (predsjednik povjerenstva)
• Committee member: Lidija Furač (član povjerenstva)
• Committee member: Vesna Ocelić Bulatović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2023-09-15, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Plastika je postala sveprisutan materijal u vodenim ekosustavima. Masovna proizvodnja dovela je i do masovnog nastajanja plastičnog otpada koji raznim procesima u prirodi postaje mikroplastični (MP) otpad te nerijetko završi upravo u vodenim staništima. Zbog dokazanih toksičnih učinaka na živi svijet MP se smatra globalnom onečišćujućom tvari u slatkovodnim i morskim ekosustavima, osobito zbog mogućnosti transporta kroz trofičke razine sve do čovjeka. Ribe su u vodi konstantno izložene riziku unosa i akumulacije MP čestica u njihov organizam. Mogu poslužiti kao dobri bioindikatori, a dosadašnja istraživanja ukazuju na visoku stopu pronalaska čestica MP-a u probavnom sustavu riba. Razvijanje metoda za izolaciju i točno kvantificiranje MP-a u morskim i slatkovodnim staništima sve je važnije. Za praćenje prostorne distribucije i vremenskih trendova morskog MP otpada, izrazito je važno razviti i implementirati standardizirane protokole, kako bi se učinkovito i točno odredila količina MP-a u različitim staništima. U ovome radu ispitivao se utjecaj razgradnje primjenom četiri različite metode na izolaciju i određivanje MP čestica iz probavila riba iz slatkovodnih i morskih ekosustava Republike Hrvatske. Dobiveni rezultati ukazuju da je kombinacija dušične kiseline, HNO3 i fluorovodične kiseline, HF najprikladnija metoda za izolaciju MP iz probavila riba, s minimalnim utjecajem na njezinu strukturu. U sadržaju probavila svih analiziranih jedinki riba identificirane su MP čestice, čime smo potvrdili njezinu prisutnost i unos u organizme vodenih ekosustava.

Abstract (english)

Plastic has become a ubiquitous material in aquatic ecosystems. Its mass production has also led to the mass formation of plastic waste, which through various processes in nature becomes microplastic (MP) waste and often ends up in aquatic habitats. Due to its proven toxic effects on living things, MP is considered a global contaminant in freshwater and marine ecosystems, especially regarding the possible transportation through trophic levels, all the way to humans. In water, fish are constantly exposed to the risk of ingestion and accumulation of MP particles in their organism. They can serve as good bioindicators, and previous research indicates a high rate of finding MP particles in the digestive system of fish. Development of methods for isolation and accurate quantification of MP in marine and freshwater habitats is increasingly important. For monitoring of the spatial distribution and temporal trends of marine MP debris, development and implementation of standardized protocols is highly important, in order to efficiently and accurately determine the quantity of MP in different habitats. In this work, the influence of digestion by four different methodologies on isolation and determination of MP particles from the intestine of fish from freshwater and marine ecosystems of the Republic of Croatia were examined. The obtained results indicate that combination of nitric acid, HNO3, and hydrofluoric acid, HF represents the most appropriate methodology for MT isolation from fish intestine, with minimum influence on its structure. The particles of MP were identified in the gut content of all analysed fish, therefore confirming its presence and uptake in aquatic organisms.

• Keywords: mikroplastika; bioindikatori; ribe; metodologija izolacije mikropalstike
• Keywords (english): microplastic; bioindicators; fish; methodology for microplastic isolation
• Language: croatian
• URN:NBN: urn:nbn:hr:149:405125
• Project: Number: IP-2019-04-9661; Title: Primjena naprednih tehnologija obrade voda za uklanjanje mikroplastike; Title: Advanced Water Treatment Technologies for Microplastics Removal; Acronym: AdWaTMiR; Leader: Tomislav Bolanča; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Project: Number: IP-2020-02-8502; Title: Integrirana procjena odgovora akvatičkih organizama na izloženost metalima: ekspresija gena, bioraspoloživost, toksičnost i biomarkerski odgovori; Title: Integrated evaluation of aquatic organism responses to metal exposure: gene expression, bioavailability, toxicity and biomarker responses; Acronym: BIOTOXMET; Leader: Vlatka Filipović Marijić; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-01-01
• Created on: 2023-09-22 08:36:12