Enizimska razgradnja polimera

undergraduate thesis

• Title (english): Enzymatic degradation of polymers
• Author: Mirjana Tolj
• Mentor: Mirela Leskovac (mentor)
• Committee member: Mirela Leskovac (predsjednik povjerenstva)
• Committee member: Zrinka Buhin Šturlić (član povjerenstva)
• Committee member: Ljerka Kratofil Krehula (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2018-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering; Chemical Engineering in Material Development

Abstract

Razgradivi polimeri se razgrađuju tijekom ili neposredno nakon njihovog perioda primjene. Biorazgradnja se može odvijati pod utjecajem mikroorganizama, enzima ili gljivica ili se može javiti isključivo zbog kemijskih ili radikalskih procesa kao što su hidroliza, oksidacija i UV zračenje. Polistiren (PS) je plastomer linearnih makromolekula koje se sastoje od niza –CH2-CH(C6H5)- ponavljanih jedinica. Poli(ε-kaprolakton) (PCL) je biorazgradivi alifatski poliester koji se široko primjenjuje za uporabu u medicinskim implantatima. PCL se polako razgrađuje zbog prisutnosti pet hidrofobnih –CH2 skupina u njegovim ponavljajućim jedinicama. U ovom radu istražen je utjecaj enzima lipaze na razgradnju poli(ε-kaprolaktona) (PS) i polistirena (PS). Praćena je promjena mase prije i tijekom enzimske razgradnje te količina apsorbirane vode, dok su površinske karakteristike istražene mjerenjem kontaktnog kuta i primjenom FTIR-ATR spektroskopije. Dobiveni rezultati pokazali su da u prisutnosti enzima lipaze dolazi do razgradnje PCL-a.

Abstract (english)

Degradable polymers will degrade throughout or instantly after their application period. Biodegradation can take place under the influence of microorganisms, enzymes or fungi or may occur due to the chemical or radical processes, such as hydrolysis, oxidation, and UV irradiation. Polystyrene (PS) is a plastomer of linear macromolecules consisting of -CH2-CH(C6H5)- repetitive units. Poly (ε-caprolactone) (PCL) is biodegradable aliphatic polyester widely used for medical implants. PCL slowly degrades due to the presence of five hydrophobic -CH2 groups in its recurring units. In this work, the effect of the enzyme lipase on the degradation of poly (ε-caprolactone) and polystyrene was investigated. Before and during enzymatic degradation the mass change and the amount of absorbed water were investigated. Surface characteristics were explored by measuring the contact angle and using FTIR-ATR spectroscopy. The obtained results show that PCL degrade in the presence of enzyme lipase.

• Keywords: polistiren; poli(ε-kaprolakton); enzimska razgradnja; lipaza
• Keywords (english): polystyrene; poly (ε-caprolactone); enzymatic degradation; lipase
• Language: croatian
• URN:NBN: urn:nbn:hr:149:821300
• Study programme: Title: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Applied organic chemistry; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-06-23 11:40:31