Imobilizacija aldolaze na magnetske nanočestice

master's thesis

• Title (english): Immobilisation of aldolase on magnetic nanoparticle
• Author: Tomislav Rimac
• Mentor: Ana Vrsalović Presečki (mentor)
• Committee member: Ana Vrsalović Presečki (predsjednik povjerenstva)
• Committee member: Ana Lončarić Božić (član povjerenstva)
• Committee member: Marin Kovačić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2019-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Enzim deoksiriboza-5-fosfat aldolaza (DERA) katalizira reakciju sinteze (3R,5R)-6-kloro-3,5- dihidroksi heksanala (laktol) dvostrukom aldolnom adicijom dvije molekule acetaldehida i jedne molekule kloroacetaldehida. Visoka koncentracija aldehida u DERA reakciji ima izrazito negativan utjecaj na stabilnost enzima. Cilj ovog rada bio je provesti imobilizaciju enzima DERA-e na magnetske nosioce u svrhu povećavanja njegove stabilnosti. Kao nosioci za imobilizaciju korištene su magnetske nanočestice obložene neporoznom i mezoporoznom silikom. Nosioci su aktivirani s benzokinonom, glutaraldehidom i epoksi skupinom. Najveća stabilnost i aktivnost dobivena je imobilizacijom enzima DERA-e na magnetske nanočestice obložene neporoznom silikom aktivirane benzokinonom.

Abstract (english)

Deoxyribose-5-phosphate aldolase (DERA) catalyses the synthesis of (3R,5R)-6-chloro-3,5-dihidroxyhexanal (lactol) by double aldol addition of two molecules of acetaldehyde and one molecule of chloroacetaldehyde. High aldehyde concentrations in DERA reaction have significant negative impact on enzyme stability. The aim of this paper was to carry out the immobilization of DERA enzyme on magnetic carriers in order to increase its stability. Magnetic nanoparticles coated with mesoporous and nonporous silica were used as the immobilisation carriers. Carriers were activated by benzoquinone, glutaraldehyde and epoxy group. The highest stability and activity was achieved by immobilising DERA enzyme onto nanomagnetic particles coated with nonporous silica and activated by benzoquinone.

• Keywords: deoksiriboza-5-fosfat aldolaza; imobilizacija enzima; aldolna adicija; deaktivacija enzima; silika; magnetske nanočestice
• Keywords (english): deoxyribose-5-phosphate aldolase; enzyme immobilisation; aldol addition; enzyme deactivation; silica; magnetic nanoparticles
• Language: croatian
• URN:NBN: urn:nbn:hr:149:106823
• Study programme: Title: Environmental Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2019-07-12
• Created on: 2022-02-27 14:40:13