Priprema i karakterizacija PLA/fibroin svile kompozita

master's thesis

• Title (english): Preparation and characterization of PLA/silk fibroin composites
• Author: Josip Kos
• Mentor: Emi Govorčin Bajsić (mentor)
• Committee member: Emi Govorčin Bajsić (predsjednik povjerenstva)
• Committee member: Ljerka Kratofil Krehula (član povjerenstva)
• Committee member: Mirela Leskovac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2022-09-08, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Polilaktidna kiselina (PLA)/fibroin svile (FS) kompozit je zbog svoje biorazgradivosti i biokompatibilnosti tijekom protekla dva desetljeća primjenjivan u industriji i biomedicini, s naglasnom na tkivnom inženjerstvu. U ovom su radu pripremljeni PLA/FS kompoziti različitih udjela FS-a te je istražen utjecaj FS-a na svojstva PLA. PLA/FS kompoziti pripremljeni su dodatkom različitog udjela FS umješavanjem u laboratorijskoj Brabender gnjetilici. Provedene tehnike za karakterizaciju PLA/FS kompozita su: diferencijalna pretražna kalorimetrija (DSC), termogravimetrijska analiza (TGA), dinamičko mehanička analiza (DMA), mjerenje kontaktnog kuta i bubrenje. Rezultati dobiveni DSC tehnikom pokazali su da se dodatkom fibroina svile temperatura taljenja pomiče na niže temperature, dok temperatura kristalizacije raste. Stupanj kristalnosti raste povećanjem udjela FS zbog nukleacijskog učinka FS. Toplinska stabilnost PLA/FS kompozita mjerena u struji dušika i zraka smanjuje se dodatkom fibroina svile. Rezultati dobiveni DMA tehnikom pokazali su da kompoziti s većim udjelom FS-a imaju veći modu pohrane pri 25 °C. Temperatura staklastog prijelaza neznatno je niža u odnosu na čisti PLA. Mjerenjem kontaktnog kuta utvrđeno je da kod kompozita s većim udjelom FS-a dolazi do smanjenja disperznih doprinosa, odnosno dolazi do porasta polarnih doprinosa. Ispitivanjem bubrenja utvrđeno je da su kompoziti s većim udjelom FS-a hidrofilniji.

Abstract (english)

Polylactic acid (PLA)/silk fibroin (FS) composite has been used in industry and biomedicine for the past two decades due to its biodegradability and biocompatibility, with an emphasis on tissue engineering. In this work, PLA/FS composites with different content of FS were prepared and the effect of SF on the properties of PLA was investigated. PLA/ SF composites were prepared with different weight content of SF via melt mixing in a laboratory Brabender mixer. Techniques used for the characterization of PLA/SF composites are: differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), dynamic mechanical analysis (DMA), contact angle measurement and swelling. Results obtained with DSC technique showed that with addition of SF, melting temperature decreases, while crystallization temperature increases. The addition of SF increased the degree of crystallinity because of the nucleation effect of SF. Thermal stability of PLA/SF composites in the nitrogen and air atmosphere decreased with addition of SF. Results obtained with DMA technique showed that composites with a higher SF content have a higher storage modulus at 25 °C. The glass transition temperature is slightly lower compared to the neat PLA. The contact angle measurements confirm that in composites with a higher SF content dispersed contributions decrease while polar contributions increases. The swelling test revealed that composites with a higher SF content are more hydrophilic.

• Keywords: polilaktidna kiselina (PLA); fibroin svile (FS); kompoziti; tkivno inženjerstvo
• Keywords (english): polylactic acid (PLA); silk fibroin (SF); composites; tissue engineering
• Language: croatian
• URN:NBN: urn:nbn:hr:149:597622
• Study programme: Title: Chemical Engineering - Graduate study; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka kemijskog inženjerstva (magistar/magistra inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-03-10 15:26:34