Priprema otapala za ekstrakciju polifenola

undergraduate thesis

• Title (english): Solvent preparation for polyphenol extraction
• Author: Anđela Mišković
• Mentor: Jasna Prlić Kardum (mentor)
• Committee member: Jasna Prlić Kardum (predsjednik povjerenstva)
• Committee member: Aleksandra Sander (član povjerenstva)
• Committee member: Dajana Kučić Grgić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2021-07-09, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Za ekstrakciju i separaciju biokomponenti, primjenjuju se organska otapala što donosi velike ekološke probleme. Kako bi se ublažio ovaj problem, pokrenuta su brojna istraživanja o otkrivanju obnovljivih zelenih otapala. Niskotemperaturna eutektička otapala (eng. Deep Eutectic Solvents-DES) i prirodna niskotemperaturna eutektička otapala (eng. Natural Deep Eutectic Solvents- NADES ) su se pokazala dobrom zamjenom za organska otapala i ionske kapljevine. Navedena otapala karakterizira netoksičnost, nezapaljivost, biorazgradljivost i niska cijena. Polifenoli su spojevi koji dobro utječu na ljudsko zdravlje te imaju velik morfološki i fiziološki značaj. U pitanju su prirodni antioksidansi koji sprječavaju razne bolesti, pozitivno djeluju na kardiovaskularne poteškoće te usporavaju starenje. U ovom radu proučena je primjenjivost DES-ova i NADES-a za ekstrakciju polifenola iz biljke trputca (lat. Plantago major) u odnosu na konvencionalna otapala. Pripremljena su 22 različita otapala kojima su karakterizirana svojstva. Prikazani su rezultati ekstrakcije polifenola, gustoća te pH vrijednost. Naglasak je na NADES-ima koje čine dvije komponente: mliječna kiselina i glicerol (LaGly) s različitim udjelima vode.Istraživanje je pokazalo da je najpogodnije otapalo za ekstrakciju polifenola iz trputca LaGly 2:1 s 10 mas.% vode.

Abstract (english)

Organic solvents are used for the extraction and separation of biocomponents, which brings major environmental problems. To reduce this problem, numerous studies have been launched to find renewable green solvents. Deep Eutectic Solvents-DES and Natural Deep Eutectic Solvents-NADES have proven to be good alternatives for organic solvents and ionic liquids. These solvents are characterized by non-toxicity, non-flammability, biodegradability and low price. Polyphenols are compounds that have good influence on human health, great morphological and physiological significance. These are natural antioxidants that prevent all kinds of diseases, have a positive effect on cardiovascular difficulties and slow down aging. In this paper, it was studied the suitability for the extraction of polyphenols from the plantain leaves (lat.Plantago major), of DES and NADES in relation to conventional solvents. 22 different solvents were prepared and characterized. The results of polyphenol extraction, density and pH value are presented. The accent is on solvents which consist of two components: lactic acid and glycerol (LaGly) with different content of water. After extraction, the amount of polyphenols in the isolated extract was analyzed. The study showed that the most suitable solvent for extraction polyphenols from the plantain leaves was LaGly 2:1 with 10% water.

• Keywords: ekstrakcija; eutektička otapala; polifenoli; trputac
• Keywords (english): extraction; eutectic solvents; polyphenols; plantain leaves
• Language: croatian
• URN:NBN: urn:nbn:hr:149:186394
• Study programme: Title: Chemical Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-04-11 08:39:27