Validacija HPLC-PDA metode za određivanje ciprofloksacina u vodi

undergraduate thesis

• Title (english): HPLC-PDA method validation for the determination of ciprofloxacin in water
• Author: Martina Vuković
• Mentor: Sandra Babić (mentor)
• Committee member: Sandra Babić (predsjednik povjerenstva)
• Committee member: Irena Škorić (član povjerenstva)
• Committee member: Martina Biošić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2021-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Nakon razvitka pojedine metode prvi slijedeći korak jest validacija iste. Validacija analitičke metode definira se kao postupak dokazivanja odnosno utvrđivanja je li metoda prikladna za uporabu u željenu svrhu. Procesu validacije metode pristupa se laboratorijskim ispitivanjima pri čemu je nužno ispitivanje izvedbenih karakteristika: selektivnosti/specifičnosti metode, linearnosti, radnoga područja, preciznosti, istinitosti, granica kvantifikacije i detekcije te robusnosti. Primarni cilj ovoga rada bio je validirati unaprijed poznatu HPLC-PDA analitičku metodu za određivanje fluorokinolonskog antibiotika ciprofloksacina. Istraživanje je provedeno u svrhu upoznavanja cjelokupnog postupka validacije analitičke metode te donošenja adekvatnih zaključaka o prihvatljivosti iste. Određene su izvedbene karakteristike metode tekućinske kromatografije visoke djelotvornosti pri čemu su same otopine ciprofloksacina pripravljane u MilliQ i riječnoj vodi. Na temelju dobivenih rezultata provedenog istraživanja potvrđena je prihvatljivost metode za određivanje ciprofloksacina u vodi, s obzirom da se dobivene vrijednosti izvedbenih karakteristika nalaze unutar definiranih granica prihvatljivosti.

Abstract (english)

After the development of a particular method, the first next step is to validate it. Validation of an analytical method is defined as the process of proving or determining whether a method is suitable for use for the desired purpose. The method validation process is approached by laboratory tests, where it is necessary to examine the performance characteristics: selectivity/specificity of the method, linearity, working range, precision, trueness, limits of quantification and detection and also robustness. The primary goal of this work was to validate an already known HPLC-PDA analytical method for the determination of fluoroquinolone antibiotic ciprofloxacin. The research was conducted to familiarize with the entire process of validation of the analytical method and to make adequate conclusions about its acceptability. Performance characteristics of the high performance liquid chromatography method with a diode array detector are determined using ciprofloxacin solutions prepared in MiliQ and river water. Based on the obtained results of the conducted research, the applicability of the method for the determination of ciprofloxacin in water was confirmed, since the obtained values of performance characteristics meet all acceptability criteria.

• Keywords: validacija analitičke metode; tekućinska kromatografija; ciprofloksacin
• Keywords (english): validation of analytical method; liquid chromatography; ciprofloxacin
• Language: croatian
• URN:NBN: urn:nbn:hr:149:717503
• Study programme: Title: Materials Science and Engineering - Undergraduate study; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-09-01
• Created on: 2023-04-27 08:05:40