Aktivnost halogenhidrin-dehalogenaze pod utjecajem organskih otapala

undergraduate thesis

• Title (english): Activity of halohydrin dehalogenase in the presence of organic solvents
• Author: Martina Čičak
• Mentor: Zvjezdana Findrik Blažević (mentor)
• Committee member: Zvjezdana Findrik Blažević (predsjednik povjerenstva)
• Committee member: Martina Sudar (član povjerenstva)
• Committee member: Marija Vuković Domanovac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2020-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

U ovome radu ispitana je aktivnost enzima halogenhidrin-dehalogenaze u prisustvu organskih otapala različitih koncentracija. Aktivnosti enzima su praćene preko produkta p-nitrofenilstiren oksida koji je dobiven pretvorbom iz 1-(p-nitrofenil)-2-bromoetanola uz halogenhidrin-dehalogenazu kao katalizator. Proučavane su relativne aktivnosti enzima budući specifične dosta odstupaju jedna od druge. Na temelju dobivenih podataka, određena je aktivnost enzima u prisustvu pojedinog organskog otapala. Aktivnost enzima opada s porastom koncentracije organskog otapala. Porastom koncentracije organskog otapala, enzimi i dalje zadržavaju aktivnost, ali ne na razini aktivnosti u vodenom mediju. U prisustvu n-heksana, cikloheksana i toluena, enzimi zadržavaju vrlo visoku aktivnost čak i u čistom organskom otapalu.

Abstract (english)

This research was focused on the activity of halohydrin dehalogenase enzyme in different concentrations of organic solvents. The assays were monitored via the p-nitrophenylstyrene oxide product obtained by conversion from 1-(p-nitrophenyl)-2-bromoethanol with halohydrin dehalogenase as catalyst. Relative activities of enzymes were evaluated and compared, since the specific ones deviate a lot from each other. Based on the obtained data, the activity of the enzyme in a particular organic solvent was determined. As concentration of organic solvent increases, enzyme activity decreases. As the concentration of the organic solvent increases, the enzymes remain active, but at lower level than in an aqueous solution. In the presence of n-hexane, cyclohexane and toluene, the enzymes retain their activity even in pure organic solvents.

• Keywords: halogenhidrin-dehalogenaza; enzim; aktivnost; kataliza; organska otapala
• Keywords (english): halohydrin dehalogenase; enzyme; activity; catalysis; organic solvents
• Language: croatian
• URN:NBN: urn:nbn:hr:149:747727
• Project: Number: IP-2018-01-4493; Title: Enzimska sinteza fluoriranih kiralnih građevnih blokova; Acronym: EnzyFluor; Leader: Maja Majerić-Elenkov; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Advanced materials and technologies; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-10-01
• Created on: 2023-05-03 10:39:35