Ekstrakcija polifenolnih spojeva iz kožice grožđa raspršenjem matice uzorka kroz čvrstu fazu

master's thesis

• Title (english): Extraction of polyphenolic compounds from grape skin by matrix solid-phase dispersion
• Author: Barbara Prišlin
• Mentor: Danijela Ašperger (mentor)
• Committee member: Danijela Ašperger (predsjednik povjerenstva)
• Committee member: Tatjana Gazivoda Kraljević (član povjerenstva)
• Committee member: Jasminka Karoglan Kontić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2015-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry; Analytic Chemistry

Abstract

Polifenoli predstavljaju skupinu spojeva biljnog podrijetla, čiju strukturu čini jedan ili više aromatskih prstena s jednom ili više hidroksilnih skupina. Uobičajena su sastavnica ljudske prehrane te se u različitim količinama nalaze u voću, povrću, vinu, voćnim sokovima, čaju i kavi. Polifenoli se u crnom grožđu nalaze u kožici, sjemenki i mesu. Sastav i količina pojedinih polifenola ovisi o sorti grožđa, klimatskim uvjetima, postupcima uzgoja vinove loze. Cilj ovog rada je optimizacija uvjeta za ekstrakciju polifenola iz kožice grožđa metodom raspršenjem matice uzorka kroz čvrstu fazu (MSPD). Cilj optimizacije metode je pronaći optimalne čimbenike za ekstrakciju, a to su udio organske faze, volumen ekstrakcijskog otapala, omjer mase sorbensa i mase uzorka, optimalni sorbens i ekstracijsko otapalo. Ekstrakti su analizirani tekućinskom kromatografijom visoke djelotvornosti s detektorom s nizom dioda i fluorescentnim detektorom. Najbolji rezultat za ekstrakciju polifenola iz kožice grožđa dobiven je s fenilsilicijevim dioksidom kao MSPD sorbensom s 10 mL smjese otapala acetonitril:voda=50:50 kao ekstrakcijskim otapalom. Najbolji omjer mase uzorak/sorbens pokazao se omjer 125 mg uzorka/375 mg sorbensa.

Abstract (english)

Polyphenols are organic compounds, which structure makes one or more aromatic rings with one or more hydroxyl groups. Polyphenols colud be find in food especially fruits, vegetables, wine, fruit juices, tea and coffee. In red grapes we can find polyphenols in grape skin, seeds and pulp. The composition and quantity of particular polyphenols depends on grape cultivar, climatic conditions, methods of grapevine growing. The aim of this work is optimization conditions for extraction of polyphenolic compounds from grape skin by matrix solid-phase dispersion (MSPD). Parameters which were optimized are percent of organic eluation solvent, volume of eluation solvent, ratio of mass sample/sorbent, sorbent and the eluation solvent. The extracts were analyzed by high-performance liquid chromatography with diode array detector (HPLC-DAD) and fluorescence detector (HPLC-FLD). The best results for extraction of polyphenolic compounds from grape skin are obtained with Phenyl as an MSPD sorbent with 10 mL of acetonitrile:water=50:50 as an elution solvent. The best ratio of mass sample/sorbent is 125 mg sample/375 mg sorbent.

• Keywords: polifenoli; kožica grožđa; ekstrakcija raspršenjem matice uzorka kroz čvrstu fazu (MSPD); tekućinska kromatografija visoke djelotvornosti
• Keywords (english): polyphenols; grape skin; matrix solid-phase dispersion (MSPD); high-performance liquid chromatography
• Language: croatian
• URN:NBN: urn:nbn:hr:149:040538
• Study programme: Title: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Environmental chemistry and green technologies; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra primijenjene kemije (magistar/magistra primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-03-06 13:55:00