Karakterizacija ekstrakata đumbira i kurkume

master's thesis

• Title (english): Characterization of ginger and turmeric extracts
• Author: Iva Blažević
• Mentor: Aleksandra Sander (mentor)
• Committee member: Aleksandra Sander (predsjednik povjerenstva)
• Committee member: Dajana Kučić Grgić (član povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2023-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering

Abstract

Iako su eterična ulja sastavni dio ljudske povijesti i kulture već dugi niz stoljeća, njihova raznolika svojstva i danas omogućavaju razvoj inovativnih proizvoda u raznim industrijskim granama, posebice u farmaceutskoj, prehrambenoj i kozmetičkoj industriji, Budući da u današnje vrijeme fokus na ekološki održive proizvode dobiva na zamahu, uloga eteričnih ulja time postaje još značajnija. Cilj ovog rada bio je provođenje kvantitativne i kvalitativne analize ekstrakata dobivenih iz rizoma biljaka Zingiber officinale i Curcuma longa te ispitivanje antimikrobne aktivnosti na bakterijama Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus i Escherichia coli, i kvascu Candida lipolytica. Provedena je karakterizacija pomoću tankoslojne kromatografije, plinske kromatografije s masenom spektrometrijom, infracrvene spektroskopije s Fourierovom transformacijom i UV/Vis spektrofotometrije. Navedenim metodama utvrđena je varijabilnost kemijskog sastava ovisno o metodi ekstrakcije, načinu sušenja i ekstrakcijskom otapalu. Disk difuzijskim testom ispitivana je antimikrobna aktivnost ekstrakata đumbira i kurkume. Utvrđeno je značajno inhibicijsko djelovanje ekstrakata kurkume na testirane mikroorganizme, dok su ekstrakti dobiveni iz đumbira demonstrirali značajno inhibicijsko djelovanje na bakteriju B. subtilis i kvasac C. lipolytica.

Abstract (english)

While essential oils have been an integral part of human history and culture for centuries, their diverse properties continue to facilitate the development of innovative products across various industrial sectors, particularly in the pharmaceutical, food, and cosmetic industries. As the focus on ecologically sustainable products gains momentum, the role of essential oils becomes even more significant. The aim of this study was to conduct a quantitative and qualitative analysis of extracts obtained from the rhizomes of Zingiber officinale and Curcuma longa plants, as well as to investigate their antimicrobial activity against the bacteria Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and the yeast Candida lipolytica. Characterization was carried out using thin-layer chromatography, gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, and UV/Vis spectrophotometry. Through these methods, the variability in chemical composition was determined, dependent on extraction method, drying technique, and solvent used for extraction. The antimicrobial activity of ginger and turmeric extracts was assessed using disk diffusion tests. A significant inhibitory effect of turmeric extracts on the tested microorganisms was observed, while extracts derived from ginger demonstrated substantial inhibitory activity against the bacterium B. subtilis and the yeast C. lipolytica.

• Keywords: Curcuma longa; ekstrakcija; eterično ulje; hidrodestilacija; Zingiber officinale
• Keywords (english): Curcuma longa; essential oil; extraction; hydrodistillation; Zingiber officinale
• Language: croatian
• URN:NBN: urn:nbn:hr:149:556025
• Study programme: Title: Applied Chemistry - Graduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Environmental chemistry and green technologies; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra primijenjene kemije (sveučilišni/a magistar/magistra primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-11-21 12:41:57