Oksidacija diola u prisustvu oksidoreduktaza

undergraduate thesis

• Title (english): Diol oxidation in the presence of oxidoreductases
• Author: Jelena Jelačić
• Mentor: Zvjezdana Findrik Blažević (mentor)
• Committee member: Zvjezdana Findrik Blažević (predsjednik povjerenstva)
• Committee member: Martina Sudar (član povjerenstva)
• Committee member: Marija Vuković Domanovac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Chemical Engineering and Technology; Zagreb
• Defense date and country: 2018-09-10, Croatia
• Scientific / art field, discipline and subdiscipline: TECHNICAL SCIENCES; Chemical Engineering; Reaction Engineering

Abstract

U ovom radu istražena je enzimatska oksidacija alkohola katalizirana enzimom galaktoza oksidazom. Zanimala nas je specifična aktivnost galaktoza oksidaze prema alkoholnom supstratu, diolu. Provedeno je mjerenje utjecaja koncentracije diola, bakrovih iona i kisika na aktivnost enzima. Kao supstrati su korišteni racemična smjesa diola, ali i čisti R- i S- izomeri diola, kako bi se pokazalo je li enzim enantioselektivan. Postojanje produkta enzimatske reakcije oksidacije alkohola ispitano je kapljevinskom kromatografijom visoke djelotvornosti i kombinirane tehnike kapljevinske kromatografije i masene spektrometrije. Provedena je i kaskadna reakcija u kojoj je oksidacija alkohola kombinirana s aldolnom adicijom uz enzim D-fruktoza-6-fosfat aldolazu (FSA).

Abstract (english)

In this work, enzymatic alcohol oxidation catalyzed by galactose oxidase was studied. We were interested in the specific activity of galactose oxidase to the alcohol substrate. Measurement of the effect of diol, copper ions and oxygen concentration on enzyme activity was performed. The racemic alcohol was tested as substrate, but also the R- and S-isomers. The existence of enzymatic reaction products was tested by high-performance liquid chromatography and combined liquid chromatography and mass spectrometry techniques. Cascade reactions of alcohol oxidation and aldol addition to D-fructose-6-phosphate aldolase (FSA) were also performed.

• Keywords: enzimatska oksidacija; galaktoza oksidaza; alkohol; specifična aktivnosti
• Keywords (english): enzymatic oxidation; galactose oxidase; alcohol; specific activity
• Language: croatian
• URN:NBN: urn:nbn:hr:149:833725
• Project: Number: 635595; Title: Sustainable industrial processes based on a C-C bond-forming enzyme platform; Acronym: CARBAZYMES; Leader: Zvjezdana Findrik Blažević; Jurisdiction: eu; Funder: EC; Funding stream: H2020
• Study programme: Title: Applied Chemistry - Undergraduate study; specializations in: Environmental chemistry and green technologies, Advanced materials and technologies, Applied organic chemistry; Course: Advanced materials and technologies; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) primijenjene kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-06-24 12:50:44